Intermediate

Part:BBa_K2172000

Designed by: Bowen Xiao   Group: iGEM16_CIEI-BJ   (2016-10-14)


GST-Thrombin-GFP

This part contains a GST label, a thrombin protease cleavage site and a GFP detector. The GST label can be used to purify proteins. The thrombin site can be used to digest the part into two. The GFP detector can verify where the part is being expressed or not. This part can be used to synthesize other parts that require to be purified and be checked for its expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 673
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


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Categories
Parameters
None